Introduction Mitochondria are involved in numerous essential cellular processes: they produce adenosine triphosphate (ATP) by oxidative phosphorylation, participate in various metabolic pathways, contribute to calcium homeostasis and signaling, and play a key role in apoptosis. Key Words: Cytochrome-c oxidase mitochondrial fusion mitochondrial morphology succinate dehydrogenase.ġ. In this chapter, we describe methods for the visualization of mitochondria and mitochondrial activity in cultured mammalian cells to establish the localization or distribution of its components and to study mitochondrial fusion. The recent past has seen the identification and characterization of the first proteins governing the organization, function, and dynamics of mitochondria and mitochondrial DNA, and it is predictable that numerous new proteins will require localization and functional characterization in the future. Spelbrink, and Manuel Rojo Summary Mitochondria form a dynamic network in which continuous movement, fusion, and division ensure the distribution and exchange of proteins and deoxyribonucleic acid (DNA). 1 The Mitochondria of Cultured Mammalian Cells I: Analysis by Immunofluorescence Microscopy, Histochemistry, Subcellular Fractionation, and Cell Fusion Florence Malka, Karine Auré, Steffi Goffart, Johannes N.
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